BOVINE IVF PROCESS

Streamlined and Effective

1

OOCYTE PICK-UP

Oocyte pick-up (OPU), or egg collection, occurs on the farm. An ultrasound guided needle is inserted into the ovaries. The oocytes are removed from the ovaries with a vacuum pump and placed into vials. These vials are then transported back to the laboratory. Typically, anywhere between 10-20 oocytes are collected during one aspiration.

2

FERTILIZATION

Once back at the lab, the oocytes are fertilized using semen from any commercially available bull. One straw of sexed semen can fertilize oocytes from 6 different donors, and a conventional unit can fertilize up to 20 donors. Sexed semen can be used to ensure that almost all (98%) of the embryos produced will be females.

3

CULTURE

Once fertilized, the embryos are moved to another media and placed into incubators to develop into blastocysts. Their progress is monitored over their 7 days of growth. Depending on semen type, oocyte quality and donor characteristics, anywhere from 20%-90% of the oocytes collected will turn into usable embryos.

4

FREEZING

If recipients are not available for fresh implants, embryos can be frozen for later use. The highest quality embryos are selected and then frozen in 1/4cc straws. Each embryo straw is labeled with all pertinent information according to IETS guidelines.

5

IMPLANT

Embryos are implanted into recipient animals, 7 or 8 days after they have been in heat. This process is similar to artificial insemination, but the embryo needs to be implanted into one of the horns of the uterus.

208-420-0490

476 Cheney Dr W

Twin Falls, ID 83301

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